Over the years, we have extensively optimized sample preparation protocols for a wide array of mouse and human tissues. Our expertise ranges from straightforward samples like blood to more complex specimens such as pancreas and prostate.
SAIL has successfully processed tumor biospecimens obtained from clinical resections, core needle biopsies, fine needle aspirates, and liquid sources. We have also invested significant effort into optimizing multi-omics approaches, such as combining single-cell RNA sequencing (scRNAseq) with single-cell ATAC sequencing (scATACseq). Through extensive collaboration, including our participation in the Human Tumor Atlas Network, we have demonstrated the feasibility of generating robust single-cell multi-omics datasets.
For instance, we have recently:
Implemented DOGMAseq, an innovative multiomics method developed at NYGC, facilitating native lineage tracing through mitochondrial DNA sampling, along with achieving higher gene expression recovery compared to the traditional 10X Genomics Multiome methods.
Partnered with S2 Genomics, utilizing their Singulator instrument to enhance throughput and consistency in nuclei preparation. Over the past year, more than 400 samples have been processed with the Singulator at SAIL for snRNAseq, snATAC, Multiome, DLP+ and Mission Bio Tapestry.
Implemented and optimized protocols to apply snRNAseq flex approach to FFPE samples, broadening the scope of single-cell analysis to include archived and fixed tissues.
Improved enrichment for fragile cell type using LeviCell EOS by LevitasBio